Single-use, TA-ligation-ready, stubby Y adapter and indexing primers
Ensure maximum accuracy in your NGS reads with xGen Stubby Adapter and Unique Dual Indexing (UDI) Primer Pairs. A convenient indexing option for a variety of applications from whole-genome to targeted sequencing, the kit provides adapters and unique i5 and i7 primer pairs, compatible with TruSeq™-compatible library prep for sequencing on Illumina instruments.
xGen Stubby Adapter and UDI Primer Pairs are designed for TA-ligation libraries, such as libraries created using the Lotus DNA Library Prep Kit. The xGen Stubby Adapter is a short Y adapter that can be ligated to fragments with A overhangs generated during library prep. Indexing primers containing unique i5 and i7 indexes as well as P5 and P7 sequences are added to the library via PCR amplification.
The kit contains the stubby adapter and indexing primer pairs with 8 base i5 and i7 indexes. Unique dual indexes (UDIs) enable identification of misassigned reads, so these can be filtered during data analysis to improve accuracy. Adapters and indexing primers are also sold separately.
|Sample index length||8 base non-redundant sample indexes|
|Number of indexes||16 or 96 unique dual indexes (UDIs) per kit|
|Concentration||15 µM (Stubby Adapter), 20 µM total (10 µM each Primer)|
|Shipping container||Tubes (Stubby Adapter), 96-well plates (UDI Primer Pairs)|
|Reactions per well||1 reaction per primer pair|
|Adapter modifications||Methylated for bisulfite sequencing*|
|QC and quantification||NGS|
|Illumina platform compatibility||2- and 4-color platforms|
* For bisulfite sequencing, see our FAQ for additional information.