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PCR / qPCR / dPCR

Custom qPCR Probes

Multiplex your way with a wide range of high-efficiency quenchers, dyes, and scales.

Your Next MasterMix

Bypass extraction, gain 5-plex sequencing, and maintain sensitivity, even with crude samples.

Gene Synthesis & Fragments

gBlocks & gBlocks HiFi Gene Fragments

eBlocks Gene Fragments

Gene Synthesis Service

Megamer ssDNA DNA Fragments

Accelerate your high-throughput screening workflows with ready-to-use, NGS-verified clonal genes.

gBlocks HiFi Gene Fragments

Reduce screening time and minimize cloning errors with the only NGS-verified clonal gene fragment.

Next Generation Sequencing

Library Preparation

DNA Library Prep Kits

xGen DNA Library Prep Kits xGen cfDNA & FFPE DNA Library Prep Kit xGen ssDNA & Low-Input DNA Library Prep Kit

RNA Library Prep Kits

xGen Broad-Range RNA Library Prep Kit xGen RNA Library Prep Kit

Methyl-Seq Library Prep Kit

Hybridization Capture

xGen Predesigned Hyb Panels

xGen Exome Hyb Panel xGen Pan-Cancer Hyb Panel xGen AML Cancer Hyb Panel xGen Minimal Residual Disease (MRD) Hyb Panel xGen Inherited Disease Hyb Panel xGen CNV Backbone Hyb Panel xGen Human ID Hyb Panel xGen Human mtDNA Hyb Panel xGen SARS-CoV-2 Hyb Panel

xGen Custom Hyb Panels

xGen Hybridization Capture Core Reagents xGen Exome Sequencing Kit Trinity for Element

Library Normalization

xGen Normalase Module

xGen Pre-hybridization Capture Normalase Module

Anchored Multiplex PCR

T & B Cell RNA Assays

Archer Analysis

Adapters & Primers for NGS Sequencers

for Illumina Sequencers

for Ultima Genomics Sequencers

for Element Biosciences Sequencers

for DNBSEQ Sequencers

Sequencing Services

Your Next MasterMix

Bypass extraction, gain 5-plex sequencing, and maintain sensitivity, even with crude samples.

Custom Hyb Panels Design

We'll help you design fast, flexible target enrichment panels that fit into your NGS workflow.

Gene Regulation

RNA interference

Antisense Oligonucleotides

miRNA inhibitors

Antisense Oligonucleotides

Increase stability and reduce toxicity with nuclease-resistant mods and flexible chimeric designs.

DsiRNAs for RNAi

Effectively target cytoplasmic mRNA and noncoding RNA with predesigned or custom RNAi solutions.

GMP Oligo Manufacturing

GMP Primers & Probes

MGB Eclipse® Probes

Custom DNA Oligos

Unlock new research paths with over 1400 modifications available.

Build Yours Now

CGMP CRISPR Manufacturing

CGMP Guide RNAs

CGMP Cas Nucleases

CGMP gRNA Manufacturing

Accelerate your CRISPR therapeutics with our full CGMP guide RNA manufacturing/regulatory services.

OEM & Additional Services

Assay Development

SciTools Plus API

PAGE & HPLC Purification

Formulation & Packaging

Custom Quality Control

Need Integration Help?

Let our team help with your e-commerce integration, punchout, portal, or API needs.

Product Savings

Custom DNA Oligos

>90% of standard desalted orders shipped within 24 hours. Quality checked by ESI-MS.

Accelerate your high-throughput screening workflows with ready-to-use, NGS-verified clonal genes.

Explore Solutions

Cancer Research

Cancer whole genome sequencing

Cancer exome sequencing

NGS cancer screening

Biomarker discovery

TMB and MSI biomarkers

Cancer liquid biopsy

Minimal residual disease research

Infectious Disease

Viral surveillance

Rare & Inherited Genetic Disease

Antibody Discovery

Protein Engineering

Cancer Research Solutions

High performance NGS solutions with flexible, efficient workflows for your research insights.

CRISPR Therapeutics eBook

Fundamental concepts, innovations, and insights about CRISPR R&D and the therapeutic landscape.

Research Techniques

gBlocks HiFi Gene Fragments

Reduce screening time and minimize cloning errors with the only NGS-verified clonal gene fragment.

Custom DNA Oligos

Unlock new research paths with over 1400 modifications available.

Build Yours Now

Get access to expert webinars, latest product updates, and promotions.

Support & Education

Frequently asked questions

User guides and protocols

Safety data sheets

Certificates of analysis

Archer support documents

Supplier information

How to: Quotes and Orders

Simplify your ordering process and quote requests with quick, easy, self-serve options.

DECODED online newsletter

More posts in DECODED

Coronavirus DECODED articles

CRISPR genome editing DECODED articles

Custom DNA & RNA Articles

Functional genomics articles

Genes and Gene fragments

Next generation sequencing articles

qPCR and PCR articles

Webinars & video tutorials

IDT Community Blog

Get access to expert webinars, latest product updates, and promotions.

Preferred service providers

Collab Network members

IDT-Argonaut MS partnership

Get access to expert webinars, latest product updates, and promotions.

Oligos, PCR & qPCR

PCR Allele Competitive Extension (PACE) genotyping

SYBR Green dye assay and PrimeTime probe assays

Locked nucleic acids

Custom DNA Oligos

Unlock new research paths with over 1400 modifications available.

Build Yours Now

Real-Time PCR handbook

Learn how to design qPCR assays like a pro, get expert data analysis tips, and more.

Get the Handbook

CRISPR workflow

CRISPR proteins

CRISPR genome editing

Alt-R CRISPR-Cas systems

CRISPR-Cas9 genome editing

CRISPR-Cas12a (Cpf1) genome editing

CRISPR delivery

CRISPR detection

CRISPR off-target detection with NGS

CRISPR-Cas9 Systems

Optimized genome editing solutions for producing on-target, double-stranded DNA breaks.

Open up CRISPR editing to additional areas in genomes.

Synthetic Biology

Antibody applications

Accelerate your high-throughput screening workflows with ready-to-use, NGS-verified clonal genes.

DNA Cloning Guide

Learn about common DNA cloning techniques, sequence design considerations, and troubleshooting tips.

Get access to expert webinars, latest product updates, and promotions.

Oligo Design & Handling

OligoAnalyzer™ Tool

Analyzer definitions

Resuspension Calculator

Dilution Calculator

PrimerQuest Tool

Custom DNA Oligos

>90% of standard desalted orders shipped within 24 hours. Quality checked by ESI-MS.

CRISPR Genome Editing

Alt-R CRISPR HDR Design Tool

Alt-R Predesigned Cas9 crRNA Selection Tool

Alt-R Custom Cas9 crRNA Design Tool

CRISPR-Cas9 Design Checker

Alt-R HDR Design Tool

rhAmpSeq Design Tool

rhAmpSeq™ CRISPR Data Analysis Tool

CRISPR-Cas9 Systems

Optimized genome editing solutions for producing on-target, double-stranded DNA breaks.

Open up CRISPR editing to additional areas in genomes.

qPCR Assay Design

PrimerQuest™ Tool

Realtime PCR Tool

PrimeTime™ Predesigned Gene Expression Assays

PrimeTime Multiplex Dye Selection

Custom qPCR Probes

Multiplex your way with a wide range of high-efficiency quenchers, dyes, and scales.

Your Next MasterMix

Bypass extraction, gain 5-plex sequencing, and maintain sensitivity, even with crude samples.

Gene Regulation and RNAi

Predesigned DsiRNA Selection Tool

RNAi Design Tool

Antisense Oligonucleotides

Increase stability and reduce toxicity with nuclease-resistant mods and flexible chimeric designs.

DsiRNAs for RNAi

Effectively target cytoplasmic mRNA and noncoding RNA with predesigned or custom RNAi solutions.

rhAmp Genotyping Design Tool

Custom qPCR Probes

Multiplex your way with a wide range of high-efficiency quenchers, dyes, and scales.

Genes & Gene Fragments

Codon Optimization Tool

gBlocks Gene Fragments Entry Tool

Genes & Gene Fragments order status

Accelerate your high-throughput screening workflows with ready-to-use, NGS-verified clonal genes.

Library Concentration Conversion Calculator

Target Capture Probe Design & Ordering Tool

xGen™ Hyb Panel Design Tool

Custom Adapter Configurator Tool

NGS Solutions Builder Tool

Assay Marketplace

Your Next MasterMix

Bypass extraction, gain 5-plex sequencing, and maintain sensitivity, even with crude samples.

NGS Experts at Your Service

Reach your research goals faster with a consultation from our leading NGS specialists.

SciTools Plus API

Get access to expert webinars, latest product updates, and promotions.

Need Integration Help?

Let our team help with your e-commerce integration, punchout, portal, or API needs.

Company

General Information

Usage, warranty & licenses

About Us

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qPCR Master Mixes & Reagents

Efficient setup, consistent results. Save time and effort with qPCR reagents you can count on — from reliable, cost-effective everyday solutions to advanced, high performance master mixes for your most challenging samples.

Home Products PCR / qPCR / dPCR Master Mixes & Reagents

PrimeTime™ Gene Expression Master Mix

Generate reliable results under either standard or fast cycling conditions. Temperature stability permits overnight benchtop experiments and cost-effective ambient temperature shipping.

PrimeTime™ One-Step 4X Broad-Range Master Mix

Bypass extraction, even from crude samples, with our ready-to-use, 4X concentrated qPCR master mix. Advanced capabilities include up to 5-plex capacity and inhibitor resistance.

PrimeTime™ One-Step RT-qPCR Master Mix

Achieve efficient amplification with our ready-to-use 2X concentrated RT-qPCR master mix. Includes hot-start DNA polymerase, reverse transcriptase, dNTPs, MgCl₂, enhancers, and stabilizers.

rhAmp™ Genotyping Master Mix & Reporter Mixes

Use with rhAmp SNP Assays for high-confidence genotyping calls. These mixes deliver enhanced allelic discrimination and signal generation.

RNase H2 Enzyme

Reduce primer-dimers and misprimed PCR products with RNase H2. Use with rhPCR primers to increase PCR precision for accurate variant identification, microbial studies, and more.

Oligo length standards

Interpret gel bands with single-stranded DNA oligo ladders. Our marker oligos are available in two formulations and are compatible with fluorescent or non-fluorescent stains.

Looking for a custom solution?

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Selecting your master mix

Choose the PrimeTime qPCR master mix that works for you with our quick comparison table.

Mastermix	2-step RT-qPCR	1-step RT-qPCR	Direct amplification from crude samples	2X	4X	Inhibitor resistant	Shipping condition
PrimeTime Gene Expression Master Mix							Ambient temperature
PrimeTime One-Step RT-qPCR Master Mix							Dry ice
PrimeTime One-Step 4X Broad-Range Master Mix							Dry ice

Resources

A qPCR master mix that can be shipped at ambient temperatures

PrimeTime One-Step 4X Broad-Range Master Mix for one-step RT-qPCR experiments

Explore other IDT solutions

Predesigned PCR-based Assays

Choose from our expansive portfolio of high performance qPCR probe- and primer-based assays for genotyping, gene expression analysis, rapid microbial identification, and more.

rhAmp™ SNP Genotyping System

Identify SNPs and indels with predesigned assays, custom design tool, and qPCR reagent mixes. Our genotyping solutions offer reliable, cost-effective results.

Custom qPCR & dPCR Probes

Leverage over 18 reporter dye and quencher combinations, custom purification, and multiple scale options. Available for RUO or GMP*-compliant applications.

*Product manufactured using a Quality Management System based on ISO 13485:2016. Purchaser is solely responsible for all decisions regarding the use of these products and any associated regulatory or legal obligations for their legal marketing. Comment end

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Trademarks contained herein are the property of Integrated DNA Technologies, Inc. or their respective owners, and may be registered in the USA and/or other jurisdictions. For specific trademark information, see www.idtdna.com/trademarks. For research use only. Not for use in diagnostic procedures. Unless otherwise agreed to in writing, IDT does not intend for these products to be used in clinical applications and does not warrant their fitness or suitability for any clinical diagnostic use. Purchaser is solely responsible for all decisions regarding the use of these products and any associated regulatory or legal obligations.