The xGen Prism DNA Library Prep Kit includes all the reagents required for End Repair, and Ligation 1 and Ligation 2 reactions. Table 1 provides additional specifications for this kit.
Table 1. Specifications, additional reagents, and equipment
||High-quality DNA, cfDNA, DNA from FFPE samples, or double-stranded cDNA
||Mechanical shearing recommended, except for highly fragmented samples like cfDNA
||Included in the kit
||32 optimized, fixed, 8 bp UMIs|
|Indexing primers (not included)
||xGen UDI Primer Pairs*
|PCR amplification reagents (not included)
||KAPA Biosystems® HiFi HotStart ReadyMix
|Compatible sequencing platforms
||Illumina® sequencing instruments
|Compatible hybrid capture blockers
||xGen Universal Blockers—TS Mix
*Contact firstname.lastname@example.org for help ordering other indexing designs or configurations.
Complete workflow for hybridization capture experiments
The xGen Prism DNA Library Prep Kit was optimized to work seamlessly with xGen hybridization capture probes and reagents (Figure 2). Whether your project requires whole exome sequencing or custom panels, IDT has the capture solutions you need.